Neurophysiology of Sleep

Dr. H N Mallick
MD, PhD, FAMS Professor Physiology
AIIMS Delhi, India
Email id: drhmallick@yahoo.com
Phone no: +91-9810755486

Which neural structures along with their neuro-transmitters produce the state of Wakefulness?2019-01-17T06:01:44+00:00

Activities in brainstem reticular and basal forebrain activating system produce the state of arousal. The Ascending Reticular Activating System (ASRS) along with its imbedded neurotransmitters systems project widely to the cortex embracing thalamus, hypothalamus and basal forebrain on the way. The dorsal ASRS pathway originates in pons and midbrain reticular formation from LDT/PPT cholinergic and glutamatergic neurons and which project to cortex through nonspecific intralaminar and midline thalamic nuclei. The ventral ASRS originates in pontine and midbrain regions and projects to the lateral and tuberomammillary (TMN) nuclei of thalamus as well as the basal forebrain.

Sleep Secrets Nonadrenergic neurons of the locus coeruleus and serotonergic neurons of the dorsal raphe contribute to both pathways and send direct projection to cortex as do TMN histaminergic and LH orexinergic neurons.

What is the role of Orexin/hypocretin in sleepwakefulness cycle?2019-01-17T05:58:26+00:00
  • Consolidate wakefulness (Increase duration of long waking bouts)- Stimulates LC, DRN, TMN.
  • Inhibit REM Sleep
  • Increase wakefulness in periods of starvation
How does suprachaismatic nucleus interact with Sleep promoting centres to promote wakefulness?2019-01-17T05:56:02+00:00

Suprachiasmatic nucleus via dorsomedial hypothalamus sends GABAergic (inhibitory neurotransmitter) projections to Ventrolateral Pre-optic area

Which neural structures along with their neuro-transmitters produce the state of NREM Sleep?2019-01-17T05:54:35+00:00

Ventrolateral Preoptic Area(VLPO). It releases GABA and Galanin and inhibits LC, DRN, TMN and Orexinergic neurons. (Flip-Flop Model)

Which neural structure is responsible for generating sleep spindles and delta waves?2019-01-17T05:53:08+00:00

Spindles are generated by activity of GABAergic Reticular and perigeniculate nucleus of Thalamus.
Delta waves are generated by activity of thalamocortical neurons.

Which Sleep factor is implicated in Homeostatic theory of Sleep?2019-01-17T05:51:45+00:00

Adenosine.

*Adenosine causes presynaptic inhibitory effects on glutamergic cortical neurons, wake active cholinergic and orexin neurons and on GABAergic projections on VLPO.
*Adenosine meditaes its sleep promoting effects through both A1 and A2A receptors.
*Stimulants such as caffeine and Theophylline counteract the sleep promoting effects of adenosine by serving as antagonists at both A1 and A2A receptors.
*Sleep deprivation cause up regulation of A1 receptors.

Which theoretical models have been proposed to explain the REM and NREM Sleep?2019-01-17T05:48:47+00:00
  • McCarley and Hobson model- Reciprocal inhibition between Cholinergic and Aminergic group of neurons
  • McCarley and Massaquoi Model (Limit cycle Model)- incorporates the Circadian influence on REM oscillator and GABAergic REM-on and REMoff neurons in addition to reciprocal inhibition between Cholinergic and Aminergic group of neurons.
  • Lu & Saper etal model (REM Flip-Flop Model)- Involves only GABAergic and Glutamatergic neurons
  • Luppi etal model- Involves GABA, Glutamate, aminergic/orexin/MCH neurons.

*Lu etal and Luppi etal models does not explain how a change in state is achieved or any explanation how NREM-REM transitions occur.

Which neuro-transmitter is responsible for producing muscle atonia in REM Sleep?2019-01-17T05:42:45+00:00

Glycine as originally proposed and now GABA too.

Which neuro-transmitter is responsible for producing EEG desynchronization in REM sleep?2019-01-17T05:41:41+00:00

Acetylcholine.

Which neural structure is responsible for PGO wave generation during REM Sleep?2019-01-17T05:40:39+00:00

Synchronized electric filed potentials in the pons, lateral geniculate nucleus and occipital cortex occur at high amplitude in the period immediately preceding the onset of REM sleep.The ponine originator lies with in LDT/PPT,(subcoereleus in cat and parabrachial areas in rats).

Which neural structure is responsible for rapid eye movements generation during REM Sleep?2019-01-17T05:39:23+00:00

Paramedian Reticular Formation.
*REM-on neurons (Glutamatergic- Paramedian Reticular Formation and Cholinergic- LDT/PPT) project to paramedian reticular formation saccade generators, which project to Colliculus to generate rapid eye movements.

Which neural structure is responsible for penile erections during REM Sleep?2019-01-17T05:37:26+00:00

According to only two available reports lateral preoptic area-lateral septal network is responsible for penile erection during REM sleep.

Which neural structure is responsible for muscle atonia during REM Sleep?2019-01-17T05:35:10+00:00

Perilocus Ceruleus alpha (Subset of Subcoeruleus area).
*In rats subcoeruleus region is called Sublaterodorsal nucleus.

What is the role of Melanin Concentrating Hormone (MCH) in REM Sleep?2019-01-17T05:33:45+00:00

MCH decreases REM sleep

Which neural structures along with their neuro-transmitters produce the state of REM Sleep?2019-01-17T05:25:40+00:00

PPT/LDT- Release Acetylcholine.

Which inflammatory mediators promote sleep?2019-01-17T05:24:27+00:00

IL-1, TNF-α, PGD2
* Somnogenic effects of IL-1 and TNF-α are mediated through IL-1 type 1 receptor and TNF 55-kDA receptor respectively.

What is the role of Melatonin in Sleep-Wake cycle?2019-01-17T05:23:32+00:00

Promotes sleep. Used to treat advanced and delayed sleep phase syndrome by shifting biological night.

What is the role of Nitric Oxide (NO) in Sleep?2019-01-17T05:21:35+00:00
  • NO promotes NREM Sleep
  • NO produced by iNOS promotes NREM Sleep
  • NO promotes NREM Sleep by release of Adenosine
2019-03-14T07:36:25+00:00